Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Alkali Scientific MOPS, Sodium Salt [3 (N Morpholino) propanesulfonic acid sodium salt], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660063.jpg-250.jpg)
Alkali Scientific MOPS, Sodium Salt [3 (N Morpholino) propanesulfonic acid sodium salt], 100 G
MOPS (3-(N-Morpholino)propanesulfonic acid sodium salt) is a widely used biochemical reagent for pH buffering in biological and biochemical applications. This 100g volume of MOPS sodium salt is ideal for maintaining stable pH levels in systems that require a buffered environment, particularly in protein and nucleic acid studies. Its stability at a pH range of 7.0–7.5 makes it ideal for enzyme reactions, cell cultures, and electrophoresis. MOPS is commonly used in molecular biology and biochemistry for experiments that require consistent pH regulation over extended periods. It is used in a variety of applications, including gel electrophoresis, enzyme assays, and protein purification protocols.
Sigma Aldrich Fine Chemicals Biosciences HEPES sodium salt solution1L
HEPES is a general-purpose zwitterionic buffer which does not bind magnesium calcium manganese(II) or copper(II) ions. HEPES has been classified as Good buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2.
Enzo Life Sciences Ada-Tyr-(Ahx)3-(Leu)3-vinyl sulfone (100µg)
A highly potent, cell-permeable proteasome inhibitor that targets all individual active subunits with comparable affinity. This extended peptide-based inhibitor can be radioiodinated, allowing detection of labeled subunits by autoradiography. Purity: ≥97%. Long Term Storage: -20°C.
![Research Products International Corp CHES [2-(N-Cyclohexylamino)ethanesulfonic acid], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-C40020-1000.0.JPG-250.jpg)
Research Products International Corp CHES [2-(N-Cyclohexylamino)ethanesulfonic acid], 1 Kilogram
CHES, also known as 2-(N-Cyclohexylamino) ethanesulfonic acid, is a chemical compound that plays a significant role in life science research, particularly in molecular biology and protein research.
CHES is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 8.6 to 10.0, making it suitable for applications requiring higher alkaline pH conditions.
CHES is also commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability in alkaline conditions make it valuable in these applications.
One of the significant advantages of CHES is its low ionic strength, which minimizes interference with biological processes and enzymatic activities.
Medchemexpress LLC Hepes hemisodium salt | 103404-87-1 | MFCD00036463 | 99.0% | 249.80 g/mol | C8H17N2O4S · 1/2 Na | 500 MG
HEPES hemisodium salt is the hemisodium salt form of HEPES, a zwitterionic buffering agent commonly used to maintain physiological pH in biological and biochemical research. It delivers reliable buffer capacity near pH 6.8-8.2 and is supplied as a solid reagent for preparation of aqueous buffers for cell culture, enzymatic assays, and protein work.
- Provides stable buffering at pH 6.8-8.2
- Low reactivity with biological systems
- High assay purity suitable for analytical applications
- Easy to dissolve for reproducible buffer preparation
- Available in convenient lab-scale pack sizes
Medchemexpress LLC HEPPS | 16052-06-5 | 99.0% | 50 G
HEPPS (EPPS) is a buffering agent with a useful pH range from 7.3 to 8.7. It reduces Aβ-aggregate-induced memory deficits and rescues cognitive deficits in mice. EPPS is orally active and penetrates the blood-brain barrier. It binds to amyloid-β aggregates and converts them into monomers, thereby reducing the formation of amyloid-β oligomers and plaques. This agent also rescues cell damage induced by amyloid-β aggregates.
- Binds to amyloid-β aggregates and converts them into monomers
- Reduces formation of amyloid-β oligomers and plaques
- Rescues cell damage induced by amyloid-β aggregates
- Improves cognitive dysfunction in APP/PS1 transgenic mice
- Reduces Aβ oligomer and plaque deposition in the brain
- Reduces glial GABA release in the brain
Medchemexpress LLC 4-morpholineethanesulfonic acid, sodium salt (1:1) | 71119-23-8 | 98.0% | 10 G
MES sodium salt (2-Morpholinoethanesulphonic acid) is an amphoteric ion buffer with a buffering capacity ranging from pH 5.5-7.0. It is classified as a Good's buffer and is widely employed in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer with buffer capacity ranging from pH 5.5-7.0.
- Good's buffer.
- Widely used in biochemistry and molecular biology experiments.
- Provides effective buffering capacity around pH 6.15.
- pKa value changes with temperature, which should be considered for experiments at different temperatures.
- Shows good buffering properties in biological experiments.
- Exhibits poor affinity with metal ions (binding constants at 20 °C and 0.1 M: Mg2+, 0.8; Ca2+, 0.8; Mn2+, 1.7), making it suitable for studies involving metal ions.
eMolecules 205535-92-8 | Ambeed | Benzyl (2-(2-(2-hydroxyethoxy)ethoxy)ethyl)carbamate | 250mg | 660585595 | A898378 | MFCD07357495 | 283.324 | C14H21NO5
Ambeed | 6-Chlorochroman-4-one | 1g | 552578805 | A142130 | 37674-72-9 | MFCD00024070 | 182.600 | C9H7ClO2
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Research Products International Corp HEPES Buffered Saline Solution 2X, 250 Milliliters
2X Formulation:
KCI: 10 mM
Na2HPO4: 1.5 mM
Dextrose: 12 mM
HEPES: 50 mM
pH: 7.0
Sigma Aldrich Fine Chemicals Biosciences HEPBS >=99% (titration) | 161308-36-7 | MFCD00677124 | 25G
HEPBS >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 266.36 | 161308-36-7 | MFCD00677124 | 25G
Medchemexpress LLC MES sodium salt | 71119-23-8 | 98.0% | 50 G
MES sodium salt | 71119-23-8 | 98.0% | 50 G
Sigma Aldrich Fine Chemicals Biosciences PIPES | 5625-37-6 | MFCD00006159 | 500g
PIPES | Purity: 99% | Mol Wt: 302.37 | 5625-37-6 | MFCD00006159 | 500g
Bioworld ADA (N-(2-acetamido)iminodiacetic acid) Buffer, 0.2M, pH 7.0, 500 mL
Synonym:ADA-Na,N-(2-Acetamido)iminodiacetic acid monosodium salt,N-(Carbamoylmethyl)iminodiacetic acid monosodium saltA zwitterionic buffer used in biological applications including protein isolation and characterizationWarning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption. Appearance: LiquidPH Value: 7.0
Gold Biotechnology Inc HEPPSO 100 g
HEPPSO is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 7.1 – 8.5. It is commonly used as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPPSO displays no toxicity for crustaceans with which it has been studied and has been deemed suitable for toxicological studies. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. HEPPSO forms a complex with Cu(II) ions so stability constants and concentrations should be taken into consideration when using this buffer in a solution containing copper ions. Though HEPPSO binds to coppers ions, it is still suitable for use with the bicinchoninic acid (BCA) assay. Diminished protein-induced color development can be accounted for or even eliminated by precipitating protein prior to reaction with bicinchoninic acid.