Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Research Products International Corp PBS [Phosphate Buffered Saline], 1X Solution, 4.5ml Pre-Filled Tubes, Sterile, 36 Tubes per Case,10ml Tube Size](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P10900-36.0.JPG-250.jpg)
Research Products International Corp PBS [Phosphate Buffered Saline], 1X Solution, 4.5ml Pre-Filled Tubes, Sterile, 36 Tubes per Case,10ml Tube Size
Ready to use sterile 1X solution, supplied in sterile pre-filled screw cap tubes. Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. Each tube contains 4.5 ml PBS 1X Sterile Solution, packaged in a sealed white cardboard freezer box with a 6 x 6 divider. PBS is supplied in 10 ml screw cap tubes that are 3.5 “ high (cap on). Each case holds 36 pre-filled tubes.
Convenient 4.5ml of PBS per tube
Sterile
36 x 4.5 ml
Discovery Life Sciences .5M POTASSSIUM PHOS BUFFER 7.4
Potassium Phosphate Buffer, P450 Enzyme Buffer, 0.5M
Hampton Research HR2-723/1.0 M BICINE pH 9.0 - 100 ml
HR2-723/1.0 M BICINE pH 9.0 - 100 ml. Please note items from this supplier are non-returnable.
Hampton Research HR2-795/1.0 M BIS-TRIS propane pH 7.0 - 100 ml
HR2-795/1.0 M BIS-TRIS propane pH 7.0 - 100 ml. Please note items from this supplier are non-returnable.
Boston Bioproducts Inc Tris-Buffered Saline-Tween, TBST - 2x1L
Bioprocess Grade - Tris-Buffered Saline-Tween TBST - 2x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Bovine Serum Albumin, BSA (5%, TBST) - 250ml
Bovine Serum Albumin BSA (5% TBST) - 250ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HEPES Buffered Saline (2X, Low Endotoxin) - 250ml
HEPES Buffered Saline (2X Low Endotoxin) - 250ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Krebs-Henseleit Buffer (with HEPES, without Calcium) - 500ml
Krebs-Henseleit Buffer (with HEPES without Calcium) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, pH 7.4) - 500ml
Phosphate Buffered Saline PBS (10X pH 7 4) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Ethanol (20%) - 2x1L
Bioprocess Grade - Ethanol (20%) - 2x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 6.8) - 500ml
Tris-HCl Buffer (1 M pH 6 8) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Molecular Biology Grade Water - 500ml
Molecular Biology Grade Water - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris Buffered Saline-Tween, TBST (10X, with 1% Tween-20, pH 7.4) - 4x1L
Tris Buffered Saline-Tween TBST (10X with 1% Tween-20 pH 7 4) - 4x1L (For Research Use and Further Manufacturing Only)